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Topic: Estradiol without derivatization (LC-MS/MS)

Hello.

I am trying to develop a UPLC-MS/MS method for quantification of Estradiol without derivatization. In out lab we use Waters Xevo TQ S and Xevo TQ S Micro. The problem is that we could not reach the target concentrations 5-10 pg/ml on standard solutions. Does anyone know if these devices are suitable for steroid analysis because we have doubts about this. In our practice we worked on various steroids and a lot of difficulties during their developing were observed. I will be really grateful if you help me.
Thank you in advance.