1 Topic by seorli

  • seorli
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Topic: Normalisation according to chosen peptide

Hi again :)

I have another question. Is it possible to make the normalisation according to one special peptide I choose, instead of the one which has the highest intensity? I know it does not seem logical but I want to use this for my internal standard (has the same amount in all samples) for to make better comparisons visually.

2 Reply by Martin

  • Martin
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Re: Normalisation according to chosen peptide

You can use the math tools inside processing panel. Simply multiply your spectra using 1/intensity of your peptide.

Martin

3 Reply by seorli

  • seorli
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Re: Normalisation according to chosen peptide

Thank you very much!

4 Reply by MALDI-ToFer

  • MALDI-ToFer
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Re: Normalisation according to chosen peptide

Dear Martin,

Is there a way to normalize the spectra to the TIC (total ion current)?
I am using MALDI-MS on very small samples and there are thus a lot of background peaks from the matrix etc., which makes it quite difficult to use the regular normalisation which focuses on the largest peak (typically a matrix peak, with variable intensities). Also this proposed approach to use a certain peak intensity of an unsaturated peak as normalisation standard isn't ideal..

Thanks a lot!