1 Topic by Alter Lab

  • Alter Lab
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Topic: Import Calibration List

I use the Bruker Pepmix as a calibrant, identical to the one in your mMass pdf. Where do I find that list and how do I import it into my calibration lists?

Thank you

2 Reply by Martin

  • Martin
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Re: Import Calibration List

You would have to define it on your own. Just go to Libraries and Reference masses. Or save the following text into *.xml and import it into Reference masses.

<?xml version="1.0" encoding="utf-8" ?>
<mMassReferenceMasses version="1.0">

  <group name="PepMix Bruker - MALDI Neg Mo">
    <reference name="Angiotensin II [M-H]-" mass="1044.527247" />
    <reference name="Angiotensin I [M-H]-" mass="1294.670247" />
    <reference name="Substance P [M-H]-" mass="1345.720847" />
    <reference name="Bombesin [M-H]-" mass="1617.807747" />
    <reference name="ACTH clip (1-17) [M-H]-" mass="2091.071647" />
    <reference name="ACTH clip (18-39) [M-H]-" mass="2463.183747" />
    <reference name="Somatostatin 28 [M-H]-" mass="3145.456447" />
  </group>

  <group name="PepMix Bruker - MALDI Pos Mo">
    <reference name="Bradykinin (1-7) [M+H]+" mass="757.399150" />
    <reference name="Angiotensin II [M+H]+" mass="1046.541792" />
    <reference name="Angiotensin I [M+H]+" mass="1296.684768" />
    <reference name="Substance P [M+H]+" mass="1347.735423" />
    <reference name="Bombesin [M+H]+" mass="1619.822341" />
    <reference name="ACTH clip (1-17) [M+H]+" mass="2093.086160" />
    <reference name="ACTH clip (18-39) [M+H]+" mass="2465.198332" />
    <reference name="Somatostatin 28 [M+H]+" mass="3147.470975" />
  </group>

  <group name="PepMix Bruker PAC - MALDI Pos Mo">
    <reference name="Bradykinin (1-7) [M+H]+" mass="757.399160" />
    <reference name="Angiotensin III [M+H]+" mass="931.514849" />
    <reference name="Angiotensin II [M+H]+" mass="1046.541792" />
    <reference name="Angiotensin I [M+H]+" mass="1296.684768" />
    <reference name="Substance P [M+H]+" mass="1347.735423" />
    <reference name="Bombesin [M+H]+" mass="1619.822341" />
    <reference name="Neurotensin [M+H]+" mass="1672.917000" />
    <reference name="Renin Substrate [M+H]+" mass="1758.932610" />
    <reference name="ACTH clip (1-17) [M+H]+" mass="2093.086160" />
    <reference name="ACTH clip (18-39) [M+H]+" mass="2465.198332" />
    <reference name="ACTH clip (1-24) [M+H]+" mass="2932.587870" />
    <reference name="Somatostatin 28 [M+H]+" mass="3147.470975" />
    <reference name="ACTH clip (7-38) [M+H]+" mass="3657.928900" />
  </group>

  <group name="ProtMix I Bruker - MALDI Neg Av">
    <reference name="Insulin [M-H]-" mass="5733.000000" />
    <reference name="Cytochrome C [M-2H]2-" mass="6179.000000" />
    <reference name="Myoglobin [M-2H]2-" mass="8475.000000" />
    <reference name="Ubiquitin I [M-H]-" mass="8564.000000" />
    <reference name="Cytochrom C [M-H]-" mass="12359.000000" />
    <reference name="Myoglobin [M-H]-" mass="16951.000000" />
  </group>

  <group name="ProtMix I Bruker - MALDI Pos Av">
    <reference name="Insulin [M+H]+" mass="5735.000000" />
    <reference name="Cytochrome C [M+2H]2+" mass="6181.000000" />
    <reference name="Myoglobin [M+2H]2+" mass="8477.000000" />
    <reference name="Ubiquitin I [M+H]+" mass="8566.000000" />
    <reference name="Cytochrom C [M+H]+" mass="12361.000000" />
    <reference name="Myoglobin [M+H]+" mass="16953.000000" />
  </group>

  <group name="ProtMix II Bruker - MALDI Neg Av">
    <reference name="Trypsinogen [M+H]+" mass="23980.000000" />
    <reference name="Protein A [M+2H]2+" mass="22304.000000" />
    <reference name="Albumin Bovine [M+2H]2+" mass="33214.000000" />
    <reference name="Protein A [M+H]+" mass="44611.000000" />
    <reference name="Albumin Bovine [M+H]+" mass="66429.000000" />
  </group>

  <group name="ProtMix II Bruker - MALDI Pos Av">
    <reference name="Trypsinogen [M+H]+" mass="23982.000000" />
    <reference name="Protein A [M+2H]2+" mass="22306.000000" />
    <reference name="Albumin Bovine [M+2H]2+" mass="33216.000000" />
    <reference name="Protein A [M+H]+" mass="44613.000000" />
    <reference name="Albumin Bovine [M+H]+" mass="66431.000000" />
  </group>

</mMassReferenceMasses>

3 Reply by Alter Lab

  • Alter Lab
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Re: Import Calibration List

Sorry for the late post, but thank you! I really appreciate it. This software is fabulous!

4 Reply by Martin

  • Martin
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Re: Import Calibration List

Thanks, I am happy that people are using it.
Martin