Topic: exact mass determination of an intact protein

Hi Martin,

This is a great and easy to use program that is much superior to the Bioanalyist 1.5 I'm using now. I can import files from Wiff format no problem and get them read in mMass.

Right now, my challenge is to deconvolute an ESI spectrum of a protein to get an accurate intact mass.

However, I can't seem to figure out how in mMass to deconvolute the charge envelope from the spectrum to get the result? It looks like this capability is in the software, but I can't figure out the steps.

Could I ask you to point me in the right direction? thanks,


(also, can't figure out how to post images to forum, or I would have included example data)


Re: exact mass determination of an intact protein

see the screencast: [url]http://www.youtube.com/watch?v=j8cVWhX-ufg&feature=youtube_gdata_player[/url]